Course Information

[2 days Course with hands-on] Code: BCT3

Workshop / Course Contents

The two days course will cover the basics of chromatography, with special emphasis on the theory and application of gas and liquid chromatography. Where appropriate, pertinent examples will be used to illustrate the importance of these two chromatographic techniques in the analysis of food and environmental samples. Sample preparation will also be covered, especially the enrichment of low-concentration analytes encountered in environmental analyses. The laboratory exercise will provide valuable hands-on experience to the participants to operate the sample preparation and analytical chromatographic equipments

• Basic of Chromatography
  
• Gas chromatography
  
• Liquid chromatography
  
• Discussion
  
• Laboratory Exercise
  

1. Gas chromatography: Analysis of Fatty acid methyl esters
2. HPLC : Analysis of caffeine in carbonated drinks
   

• Sample preparation for Chromatography
  a. Liquid-liquid extraction (LLE)
  b. Soxhlet extraction
  c. Solid phase extraction (SPME)
  d. Solid phase microextraction (SPE)

1. Application of Chromatographic Technique in Environmental Analysis
   a. Volatile Analytes (eg. benzene, toluene, ethylbenzene, and xylenes (BTEX))
   b. Semivolatile Analytes (pesticides, aromatic hydrocarbons, herbicides)
2. Quantitative Analysis in Chromatography
   a. External standard method
   b. Internal standard method
   c. Calibration curve
   

• Laboratory Exercise

1. Analysis of hydrocarbons in common fuels in soil by solid-phase microextraction (SPME) and gas chromatography with mass spectrometry detector (GC-MSD)
   Requirements:
   a. SPME Apparatus
   The SPME apparatus consists of a fibre holder and a fibre assembly, and PDMS SPME fibre.
   b. GC with mass spectrometry detector
2. Analysis of polycyclic aromatic hydrocarbons (PAHs) in water by solid phase extraction (SPE) and liquid chromatography.
   Requirements:
   a. SPE apparatus and C18 cartridges
   b. HPLC with fluorescence detector
   
   

Trainers' Biodata

Dr Maaruf Abd Ghani  

Professor Dr Musa Ahmad

Musa Ahmad starts his carrier at UKM as tutor in May 1986 after completing his first degree in Chemistry at UKM with first class honours. He was appointed as a lecturer in December 1987 after finishing his MSc study at The University of Manchester, England. He continued his PhD at the same university in 1991 under sponsorship of Association of Commonwealth Universities. He was promoted to Associate Professor in January 1998. He was appointed as UKM Matriculation Chemistry Programme Coordinator (1994 - 1996), Head of Chemistry Programme (2002 – 2004) and Chairman for School of Chemical Sciences & Food Technology (2004 – 2007). He was promoted to Professor in August 2005. Currently he is the Deputy Dean (Undergraduate, Student Affairs & Alumni) at Faculty of Science and Technology, UKM since July 2007.

As an academician, he has shown an excellence progress in teaching, supervision, publication, research and consultancy. For the past 20 years he has involved in teaching, both for undergraduate and postgraduate level in the area of analytical chemistry (mainly optical spectroscopy and separation methods). He was awarded with Excellent Teaching Award by UKM in October 2004. For supervision, he has been supervising students at various level such as post-doctoral (2), PhD (12), MSc (33) and BSc (55). To date, he has involved in about 15 consultancy works with various agencies such as Petronas, Berjaya Group and ProLintas.

Up to date, he has published more than 150 research articles in the form of books, journal papers and conferences proceedings. In research, his focus is mainly on the area of Optical Chemical Sensors. So far he has completed more than 10 researches. The finding from his research has won almost 20 awards either at national or international levels which includes 2 gold, 1 silver and 2 bronze medals obtained during International Invention Exhibition at Geneva, Switzerland and Innova Exhibition, Brussel, Belgium. Some of these finding has been highlighted at local newspaper and TV station. He has received invitation to give plenary lectures at several conferences either at national or international levels. He has also produced 3 research patents to date. He was awarded with Young Researcher Award in October 2002 by UKM.

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