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Antioxidant Activity of Extracts and Astilbin from the Root of Smilax glabra of Vietnam
T. T. V. Trinh, V. C. Vu, T. H. Pham, V. C. Pham and Q. V. Nguyen

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ABSTRACT

Astilbin, a main component of Smilax glabra Roxb roots of Vietnam was isolated. The structure of astilbin was elucidated by NMR and mass spectra. The astilbin in extracts was analyzed by HPLC, with the content of 21.8% in ethanol extract and 41.5% in ethyl acetate extract. The antioxidant capacity of extracts was evaluated by methods: 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay, thiobarbituric acid-reactive sepecies (TBARS) assay for inhibition of lipid peroxidation via TBARS formed from Fenton system-injured mouse brain, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay for hepatoprotective effect via H2O2-injured mouse hepatocytes. The results indicated ethyl acetate extract with highest content of astilbin showed the most antioxidant capacity in DPPH (SC50: 24.9 µg/ml), TBARS (IC50 9.45) and MTT (ED50: 25.25 µg/ml).

Key words: Smilax glabra Roxb; astilbin; antioxidant activity; DPPH; MDA; MTT; lipid peroxidation.

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